More Information Product Details Synonyms Product Type Properties Formula MW CAS Source/Host Chemicals Purity Chemicals Appearance Solubility Identity Declaration Other Product Data InChi Key Shipping and Handling Shipping Short Term Storage Long Term Storage Handling Advice Use/Stability Documents MSDS Product Specification Sheet Datasheet
2,7-Dichlorofluorescein diacetate; Diacetyldichlorofluorescein
Chemical
C24H14Cl2O7
485.27
2044-85-1
Synthetic.
≥95% (HPLC)
White powder.
Soluble in DMSO or acetone.
Determined by NMR.
Manufactured by Chemodex.
Click here for Original Manufacturer Product DatasheetOur product description may differ slightly from the original manufacturers product datasheet.
VQVUBYASAICPFU-UHFFFAOYSA-N
AMBIENT
+20°C
+4°C
Keep cool and dry.Protect from light and moisture.
Stable for at least 2 years after receipt when stored at +4°C.
No
Download PDF
A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2’,7’-dichlorofluorescein (DCF). Spectral data: λex=504nm, λem=524nm in 0.1 M Tris pH 8.0 (esterase).Product References(1) A.R. Rosenkranz, et al.; J. Immunol. Meth. 156, 39 (1992) | (2) T. Takanashi, et al.; Invest. Ophthalmol. Vis. Sci. 38, 2721 (1997) | (3) E. Eruslanov & S. Kusmartsev; Methods Mol. Biol. 594, 57 (2010)